Evaluation of techniques for isolation of group A streptococci from throat cultures

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Evaluation of techniques for isolation of group A streptococci from throat cultures.

In the first study, selective sulfamethoxazole-trimethoprim blood agar (SXT-BA) and conventional blood agar (BA) plates incubated under CO2 and anaerobically were compared for their ability to recover group A streptococci from throat cultures. Recovery rates were: SXT-BA (anaerobic), 100%; SXT-BA (CO2), 98%; BA (anaerobic), 89.2%; and BA (CO2), 76.5%. Primary plate bacitracin test results could...

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Streptococci from Throat Cultures

A test employing three fluorogenic 4-methylumbelliferyl substrates and the lectin of Dolichos biflorus was developed for the identification of beta-hemolytic streptococcal colonies associated with throat cultures. This non-serological method is unique in that it permits the accurate identification of groups C, F, and G streptococci, as well as groups A and B streptococci. The method is rapid, s...

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Evaluation of anaerobic incubation for recovery of group A streptococci from throat cultures.

No statistical differences were found in the recovery of group A streptococci from throat culture specimens after overnight incubation of blood agar plates in 5% CO2 compared with anaerobiosis. Anaerobic incubation required many more subcultures and resulted in considerably greater technical time and expense.

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Virulence potential of Group A streptococci isolated from throat cultures of children from north India

BACKGROUND & OBJECTIVES Rheumatic fever (RF)/rheumatic heart disease (RHD) caused by Group A streptococcus (GAS) are more prevalent in north India as compared to the western world, where invasive diseases are common. This could be due to variation in the virulence of GAS in different geographic locations. Hence, we studied the virulence potential of GAS isolated from the throat of children from...

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Effect of atmosphere and duration of incubation on primary isolation of group A streptococci from throat cultures.

The optimal incubation conditions for isolation of group A streptococci from throat cultures are controversial. Therefore, we compared the effects of aerobic and anaerobic incubations after 24 and 48 h on the recovery of group A streptococci. Throat swabs submitted to the clinical laboratory were inoculated onto duplicate 5% sheep blood agar plates, incubated aerobically or anaerobically (GasPa...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1981

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.13.5.891-894.1981